Altered SK3/KCa2.3-mediated migration in adenomatous polyposis coli (Apc) mutated mouse colon epithelial cells.

Marie Potier-Cartereau (1) , Truong-An Tran (2) , Aurélie Chantôme (1) , Alban Girault (1) , Virginie Joulin (3) , Philippe Bougnoux (1) , Christophe Vandier (1) , Fabrice H.F. Pierre (4)

1 N2Cox - Niche, Nutrition, Cancer et métabolisme oxydatif
2 iBraiN - Imaging, Brain & Neuropsychiatry
3 GC (FRE2939) - Génomes et cancer
4 Xénobiotiques

Lost of adenomatous polyposis coli gene (Apc) disturbs the migration of intestinal epithelial cells but the mechanisms have not been fully characterized. Since we have demonstrated that SK3/KCa2.3 channel promotes cancer cell migration, we hypothesized that Apc mutation may affect SK3/KCa2.3 channel-mediated colon epithelial cell motility. We report evidence that SK3/KCa2.3 channel promotes colon epithelial cells motility. Following Apc mutation SK3/KCa2.3 expression is largely reduced leading to a suppression of the SK3/KCa2.3 channel mediated-cell migration. Our findings reveal a previously unknown function of the SK3/KCa2.3 channel in epithelial colonic cells, and suggest that Apc is a powerful regulator SK3/KCa2.3 channel.

Keywords: Animals; Cell Movement; Mice; Small-Conductance Calcium-Activated Potassium Channels

Keywords: Adenomatous Polyposis Coli Protein; Cell Line; Colon; Intestinal Mucosa; Mutant Strains

Domains: Cancer

Marie Potier-Cartereau, Truong-An Tran, Aurélie Chantôme, Alban Girault, Virginie Joulin, et al.. Altered SK3/KCa2.3-mediated migration in adenomatous polyposis coli (Apc) mutated mouse colon epithelial cells.. Biochemical and Biophysical Research Communications, 2010, 397 (1), pp.42-7. ⟨10.1016/j.bbrc.2010.05.046⟩. ⟨inserm-00662363⟩