The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes

Kim Tiedemann (1) , Chantal Iobbi-Nivol (2) , Silke Leimkühler (1)

1 University of Potsdam = Universität Potsdam
2 BIP - Bioénergétique et Ingénierie des Protéines

The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.

Mots clés: bis-MGD; chaperone; molybdenum cofactor; TMAO reductase

Domaines: Sciences du Vivant [q-bio]

Kim Tiedemann, Chantal Iobbi-Nivol, Silke Leimkühler. The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes. Molecules, 2022, 27 (9), pp.2993. ⟨10.3390/molecules27092993⟩. ⟨hal-03844841⟩