Proof of the resolution-doubling of Random Illumination Microscopy using the variance of the speckled images
Résumé
Owing to minor modifications of the optical setup, Random Illumination Microscopy (RIM) surpasses the resolution limit of a standard fluorescence microscope. RIM uses speckle illuminations of the sample to derive a single variance image from the resulting diffraction-limited acquisitions. Variancematching iterations then produce a super-resolved estimate of the sample. Here, we demonstrate that in the noiseless case, variancematching yields a unique solution for the set of spatial frequencies corresponding to a doubled resolution limit. A similar result was already proven for covariance-matching, but covariancebased iterations are not implementable in practice, due to the huge size of the covariance matrix and to the induced numerical complexity. Our new identifiability result is a strong theoretical evidence supporting the super-resolution capability of the variance-matching version of RIM.
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