AOTF-based hyperspectral imaging phase microscopy

Konstantin B. Yushkov (1) , Justine Champagne (2, 3, 4) , Jean-Claude Kastelik (3, 2, 4, 5, 6) , Oleg Yu. Makarov (1) , Vladimir Ya. Molchanov (1)

1 MISIS - National University of Science and Technology
2 OPTO - IEMN - Optoélectronique - IEMN
3 IEMN - Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
4 IEMN-DOAE - Institut d’Électronique, de Microélectronique et de Nanotechnologie - Département Opto-Acousto-Électronique - UMR 8520
5 UPHF - Université Polytechnique Hauts-de-France
6 INSA Hauts-De-France - INSA Institut National des Sciences Appliquées Hauts-de-France

Phase imaging microscopy with incoherent object illumination is convenient and affordable for biomedical research and clinics since it provides easy integration with a variety of bright-field optical microscopes. We report the design of a new hyperspectral imaging system based on a combination of a spatial light modulator (SLM) and an acousto-optic tunable filter (AOTF) for phase imaging microscopy. Contrast of phase-only objects originates from matched spectral and spatial filtering performed by the SLM and the AOTF located in Fourier-conjugate optical planes in the back-end of the optical system. The system is designed as an add-on to a standard optical microscope with incoherent diascopic sample illumination. (c) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Domaines: Sciences de l'ingénieur [physics]

Konstantin B. Yushkov, Justine Champagne, Jean-Claude Kastelik, Oleg Yu. Makarov, Vladimir Ya. Molchanov. AOTF-based hyperspectral imaging phase microscopy. Biomedical optics express, 2020, 11 (12), pp.7053-7061. ⟨10.1364/BOE.406155⟩. ⟨hal-03321563⟩