Dextran-covered pH-sensitive oily core nanocapsules produced by interfacial Reversible Addition-Fragmentation chain transfer miniemulsion polymerization

Laura Marcela Forero Ramirez (1) , Ariane Boudier (2) , Caroline Gaucher (2) , Jerome Babin (1) , Alain Durand (1) , Jean-Luc Six (1) , Cécile Nouvel (1)

1 LCPM - Laboratoire de Chimie Physique Macromoléculaire
2 CITHEFOR - Cibles thérapeutiques, formulation et expertise pré-clinique du médicament

Aiming to prepare oily core pH-sensitive nanocapsules (NCs) for anticancer drugs delivery, the use of a dextran-based transurf (DexN3-τCTAγ) as both stabilizer and macromolecular chain transfer agent in methyl methacrylate/2-(diethylamino)ethyl methacrylate (MMA/DEAEMA) miniemulsion copolymerization was investigated. NCs of about 195 nm with an oily-core of Miglyol 810 (M810) and a dextran coverage covalently linked to the poly(MMA-co-DEAEMA) intern shell have been obtained. Compared to the non-sensitive PMMA-based NCs (prepared in a similar way), these novel objects were shown to swell in acidic media and to trigger Coumarin 1 release in physiological relevant pH range. As a starting point of NCs biological effects, cytotoxicity and NCs-proteins interactions studies were performed with both PMMA and poly(MMA-co-DEAEMA)-based NCs. Finally, free azide functions from dextran-based coverage were successfully exploited to attach fluorescent model dyes to NCs surface. The overall results suggest that this novel NCs platform could be potentially used as drug nanocarriers for intravenous injection.

Domains: Chemical Sciences

Laura Marcela Forero Ramirez, Ariane Boudier, Caroline Gaucher, Jerome Babin, Alain Durand, et al.. Dextran-covered pH-sensitive oily core nanocapsules produced by interfacial Reversible Addition-Fragmentation chain transfer miniemulsion polymerization. Journal of Colloid and Interface Science, 2020, 569, pp.57-67. ⟨10.1016/j.jcis.2020.02.066⟩. ⟨hal-02651686⟩