Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope

Olivier O. Hamant (1) , Pradeep Das (1) , Agata Burian (2)

1 RDP - Reproduction et développement des plantes
2 Department of Biophysics and Morphogenesis of Plants

Analysis of meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed for an unprecedented accumulation of data on the biology of meristematic cells, as well as a better understanding of the molecular and biophysical mechanisms behind shape changes in this tissue. Here we describe in detail how to prepare shoot apices of both Arabidopsis and tomato, in order to image them over time using a confocal microscope equipped with a long distance water-dipping lens.

Keywords: Confocal laser scanning microscopy; Time-lapse and live imaging; Shoot apical meristem; Development; Cell growth

Domains: Cellular Biology

Olivier O. Hamant, Pradeep Das, Agata Burian. Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope. Plant Cell Morphogenesis: Methods and Protocols, 1992, pp.257-268, 2019, 978-1-4939-9469-4, 978-1-4939-9468-7. ⟨10.1007/978-1-4939-9469-4_17⟩. ⟨hal-02526583⟩