Total Chemical Synthesis of All SUMO-2/3 Dimer Combinations

Jennifer Bouchenna (1) , Magalie Sénéchal (1) , Hervé Drobecq (1) , Jérôme Vicogne (1) , Oleg Melnyk (1)

1 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204

One hallmark of protein chemical synthesis is its capacity to access proteins that living systems can hardly produce. This is typically the case for proteins harboring post-translational modifications such as ubiquitin or ubiquitin-like modifiers. Various methods have been developed for accessing polyubiquitin conjugates by semi- or total synthesis. Comparatively, the preparation of small-ubiquitin-like modifier (SUMO) conjugates, and more particularly of polySUMO scaffolds, is much less developed. We describe hereinafter a synthetic strategy for accessing all SUMO-2/3 dimer combinations.

Mots clés: Protein chemical synthesis; convergent; SUMO dimers; SUMO-2; SUMO-3; selective desulfurization

Domaines: Chimie organique; Chimie thérapeutique

Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, Oleg Melnyk. Total Chemical Synthesis of All SUMO-2/3 Dimer Combinations. Bioconjugate Chemistry, 2019, 30 (11), pp.2967-2973. ⟨10.1021/acs.bioconjchem.9b00661⟩. ⟨hal-02386071⟩