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Virus and cystic fibrosis: Rhinoviruses are associated with exacerbations in adult patients

Abstract : Background Few studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated. Method Sixty-four sputum samples collected from 46 adult patients were included in the study: 33 samples were collected during exacerbation of CF, and 31 during the stable phase. After extraction, nucleic acids were tested for the presence of respiratory viruses. When rhinovirus (HRV) was detected, the 5′UTR and VP4/2 regions were sequenced, and phylogenetically analyzed. The characteristics of patients in exacerbation and stable phase were compared. Results Viruses were found in 25% of samples. The HRV viruses were the most frequently detected followed by coronaviruses. Only the HRV detection was significantly associated with the occurrence of CF pulmonary exacerbation (p < 0.027). Characterization of 5′UTR and VP4/2 regions of the HRV genome specified that HRV-A, -B, -C were detected. All HRV-C were recombinant HRV-Ca. Conclusions HRV were the most frequently detected viruses; their detection was significantly associated with the occurrence of an exacerbation. The reality of viral recombination between HRV was demonstrated in CF patients for the first time, raising the role of viruses in lung microbiota. Further studies are now warranted to decipher virus impact in CF
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https://hal.archives-ouvertes.fr/hal-02266763
Contributor : Anne Goffard Connect in order to contact the contributor
Submitted on : Friday, August 16, 2019 - 10:10:05 AM
Last modification on : Tuesday, October 19, 2021 - 11:56:31 PM

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Anne Goffard, Valérie Lambert, Julia Salleron, Stéphanie Herwegh, Ilka Engelmann, et al.. Virus and cystic fibrosis: Rhinoviruses are associated with exacerbations in adult patients. Journal of Clinical Virology, Elsevier, 2014, 60 (2), pp.147-153. ⟨10.1016/j.jcv.2014.02.005⟩. ⟨hal-02266763⟩

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