Notch regulation of myogenic versus endothelial fates of cells that migrate from the somite to the limb

Alicia Mayeuf-Louchart (1) , Mounia Lagha (1) , Anne Danckaert (2) , Didier Rocancourt (1) , Frédéric Relaix (1) , Stéphane D. Vincent (3, 1) , Margaret E Buckingham (1)

1 BGMCD - Bases Génétiques, Moléculaires et Cellulaires du Développement
2 PFID - Imagerie Dynamique (Plate-Forme)
3 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

Significance During embryonic development, multipotent stem cells progressively acquire specific cell fates. The somite is an embryological structure that gives rise to different mesodermal cell types, including skeletal muscle and vascular cells of blood vessels. We show by genetic manipulation that the Notch signaling pathway promotes a vascular cell-fate choice at the expense of skeletal muscle in the mouse somite. Pax3+ cells in the adjacent somites give rise to myogenic and endothelial cells in the limbs. Gain-of-function or inhibition of Notch signaling affects this cell-fate choice prior to the migration of these somite-derived cells into the limb. This embryological role of Notch is of potential therapeutic relevance to deriving stem cells for tissue repair.

Domaines: Embryologie et organogenèse; Biologie moléculaire; Génétique animale

Alicia Mayeuf-Louchart, Mounia Lagha, Anne Danckaert, Didier Rocancourt, Frédéric Relaix, et al.. Notch regulation of myogenic versus endothelial fates of cells that migrate from the somite to the limb. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (24), pp.8844-8849. ⟨10.1073/pnas.1407606111⟩. ⟨hal-02130260⟩