Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular cartilage

Caroline Bissardon (1, 2) , Olivier Proux (3) , Sarah Bureau (1) , Elke Suess (4) , Lenny Winkel (4) , Steve Conlan (2) , Lewis Francis (2) , Ilyas Khan (2) , Laurent Charlet (1) , Jean-Louis Hazemann (5) , Sylvain Bohic (6, 7)

1 ISTerre - Institut des Sciences de la Terre
2 School of Medicine, Swansea University
3 OSUG - Observatoire des Sciences de l'Univers de Grenoble
4 IBP - Institute of Biogeochemistry and Pollutant Dynamics [ETH Zürich]
5 NEEL - MRS - Matériaux, Rayonnements, Structure
6 RSRM - Rayonnement Synchrotron et Recherche Medicale
7 ESRF - European Synchrotron Radiation Facility

The speciation of highly-diluted elements by X-ray absorption spectroscopy in a diverse range of materials is extremely challenging, especially in biological matrices such as articular cartilage. Here we show that using a high energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) technique coupled to an array of crystal analyzers, selenium speciation down to 400 ppb (μg/kg) within articular cartilage can be demonstrated. This is a major advance in the speciation of highly-diluted elements through X-ray absorption spectroscopy and opens new possibilities to study the metabolic role of selenium and other elements in biological samples.

Domaines: Imagerie

Caroline Bissardon, Olivier Proux, Sarah Bureau, Elke Suess, Lenny Winkel, et al.. Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular cartilage. Analyst, 2019, 144 (11), pp.3488-3493. ⟨10.1039/C9AN00207C⟩. ⟨hal-02087768⟩