Immuno-Glyco-Imaging in Plant Cells: Localization of Cell Wall Carbohydrate Epitopes and Their Biosynthesizing Enzymes

Marie-Laure Follet-Gueye (1) , Jean-Claude Mollet (1) , Maïté Vicré-Gibouin (1) , Sophie Bernard (1) , Laurence Chevalier (2) , Barbara Plancot (1) , Flavien Dardelle (1) , Yasmina Ramdani (1) , Sylvia Coimbra (3) , Azeddine Driouich (1)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 GPM - Groupe de physique des matériaux
3 Universidade do Porto = University of Porto

In this chapter, we describe a number of sample preparation and immunostaining procedures devoted to i) localization of cell wall heteropolysaccharides and proteoglycans within the cell wall domains and endomembrane system (Golgi stacks and associated vesicles) in various plant cell types and to ii) localization/mapping of Golgi-enzymes (glycosyltransferases) responsible for complex polysaccharide assembly within Golgi cisternae. We present procedures and examples of epitope localization using epifluorescence/confocal microscopy as well as immunogold electron microscopy.

Domaines: Botanique; Biochimie, Biologie Moléculaire

Marie-Laure Follet-Gueye, Jean-Claude Mollet, Maïté Vicré-Gibouin, Sophie Bernard, Laurence Chevalier, et al.. Immuno-Glyco-Imaging in Plant Cells: Localization of Cell Wall Carbohydrate Epitopes and Their Biosynthesizing Enzymes. Hesam Dehghani. Applications of Immunocytochemistry, INTECH, pp.297-320, 2012, ⟨10.5772/35313⟩. ⟨hal-02052017⟩