Emergence and dissemination of a linezolid-resistant Staphylococcus capitis clone in Europe

Marine Butin (1, 2) , Patricia Martins-Simões (1, 2, 3) , B. Pichon (4) , D. Leyssene (5) , S. Bordes-Couecou (5) , Hélène Meugnier (1, 2, 3) , C. Rouard (6) , N. Lemaitre (7) , F. Schramm (8) , A. Kearns (4) , I. Spiliopoulou (9) , H.-L. Hyyryläinen (10) , Oana Dumitrescu (1, 2, 3) , François Vandenesch (1, 2, 3) , Céline Dupieux (1, 2, 3) , François Laurent (1, 2, 3)

1 StaPath - Pathogénie des Staphylocoques – Staphylococcal Pathogenesis
2 Laboratoire de Bactériologie de l'Hôpital de la Croix-Rousse
3 Centre National de Reference des Staphylocoques
4 Public Health England [London]
5 CHCB - Centre Hospitalier de la Côte Basque
6 Hôpital Antoine Béclère
7 CIIL - Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204
8 Virulence bactérienne précoce : fonctions cellulaires et contrôle de l'infection aiguë et subaiguë
9 University of Patras
10 National Institute for Health and Welfare [Helsinki]

Objectives: We investigated the epidemiological, clinical, microbiological and genetic characteristics of linezolid-resistant (LZR) Staphylococcus capitis isolates from French ICUs, and compared them with LZR S. capitis isolates from other European countries. Methods: All LZR isolates were subjected to antimicrobial susceptibility testing (AST) and the presence of cfr and optrA genes as well as mutations in the 23S rRNA and ribosomal proteins were investigated using specific PCR with sequencing. The genetic relationship between isolates was investigated using PFGE and WGS. Epidemiological data concerning LZR S. capitis were collected retrospectively in French microbiology laboratories. Results: Twenty-one LZR isolates were studied: 9 from France, 11 from Greece and 1 from Finland. All were resistant to methicillin and aminoglycosides. In addition, this unusual AST profile was identified in S. capitis isolates from seven French hospitals, and represented up to 12% of the S. capitis isolates in one centre. A G2576T mutation in 23S rRNA was identified in all isolates; cfr and optrA genes were absent. All isolates belonged to the same clone on the basis of their PFGE profiles, whatever their geographical origin. WGS found at most 212 SNPs between core genomes of the LZR isolates. Conclusions: We identified and characterized an LZR S. capitis clone disseminated in three European countries, harbouring the same multiple resistance and a G2576T mutation in the 23S rRNA. The possible unrecognized wider distribution of this clone, belonging to a species classically regarded as a low-virulence skin colonizer, is of major concern not least because of the increasing use of oxazolidinones.

Mots clés: Adult; Aged; Anti-Bacterial Agents; Cluster Analysis; Drug Resistance; Bacterial; Electrophoresis; Gel; Pulsed-Field; Finland; France; Genes; Genome; Genotype; Greece; Humans; Linezolid; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Epidemiology; Molecular Typing; Mutation; RNA; Ribosomal; 23S; Sequence Analysis; DNA; Staphylococcal Infections; Staphylococcus; Young Adult

Domaines: Immunologie; Bactériologie; Virologie

Marine Butin, Patricia Martins-Simões, B. Pichon, D. Leyssene, S. Bordes-Couecou, et al.. Emergence and dissemination of a linezolid-resistant Staphylococcus capitis clone in Europe. Journal of Antimicrobial Chemotherapy, 2017, 72 (4), pp.1014-1020. ⟨10.1093/jac/dkw516⟩. ⟨hal-01911046⟩