Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols
Résumé
Gold screen-printed electrodes coated with thin films of layered double hydroxides containing cobalt and aluminium (Co 1.57 Al(OH) x SO 4 , shortened as CoAl) have been investigated for the design of an electrochemical tyrosinase-based biosensor used for the detection of a complex mixture of polyphenols extracted from green tea. Physicochemical analyses show that the resulted biosensor exhibits very attractive characteristics: a high sensitivity, a large dynamic range (up to 1000 ng.mL −1), and very low limits of detection (0.33 pg.mL −1 and 0.03 pg.mL −1 for oxidation and reduction, respectively).
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