Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction

Charline J. J. Gerard (1) , Gilles Ferry (2) , Laurent M Vuillard (2) , Jean A Boutin (2) , Léonard M.G. Chavas (3) , Tiphaine Huet (3) , Nathalie A Ferte (1) , Romain A Grossier (1) , Nadine Candoni (1) , Stéphane Veesler (1)

1 CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
2 Institut de Recherches Servier
3 SSOLEIL - Synchrotron SOLEIL

We used a microfluidic platform to address the problems of obtaining diffraction quality crystals and crystal handling during transfer to the X-ray diffractometer. We optimize crystallization conditions of a pharmaceutical protein and collect X-ray data both in situ and ex situ.

Domaines: Science des matériaux [cond-mat.mtrl-sci]; Biologie structurale [q-bio.BM]

Charline J. J. Gerard, Gilles Ferry, Laurent M Vuillard, Jean A Boutin, Léonard M.G. Chavas, et al.. Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction. Acta crystallographica Section F : Structural biology communications [2014-..], 2017, 73 (10), pp.574 - 578. ⟨10.1107/S2053230X17013826⟩. ⟨hal-01493598v2⟩