Skip to Main content Skip to Navigation
Journal articles

The General Stress Response σS Is Regulated by a Partner Switch in the Gram-negative Bacterium Shewanella oneidensis

Abstract : Here, we show that a partner-switching system of the aquatic Proteobacterium Shewanella oneidensis regulates post-translationally σS (also called RpoS), the general stress response sigma factor. Genes SO2118 and SO2119 encode CrsA and CrsR, respectively. CrsR is a three-domain protein comprising a receiver, a phosphatase, and a kinase/anti-sigma domains, and CrsA is an anti-sigma antagonist. In vitro, CrsR sequesters σS and possesses kinase and phosphatase activities toward CrsA. In turn, dephosphorylated CrsA binds the anti-sigma domain of CrsR to allow the release of σS. This study reveals a novel pathway that post-translationally regulates the general stress response sigma factor differently than what was described for other proteobacteria like Escherichia coli. We argue that this pathway allows probably a rapid bacterial adaptation.
Document type :
Journal articles
Complete list of metadatas

https://hal-amu.archives-ouvertes.fr/hal-01415795
Contributor : Laure Azzopardi <>
Submitted on : Tuesday, December 13, 2016 - 3:49:07 PM
Last modification on : Thursday, January 25, 2018 - 6:26:02 PM

Links full text

Identifiers

Collections

Citation

Sophie Bouillet, Olivier Genest, Cecile Jourlin-Castelli, Michel Fons, Vincent Méjean, et al.. The General Stress Response σS Is Regulated by a Partner Switch in the Gram-negative Bacterium Shewanella oneidensis. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2016, pp 26151-26163. ⟨10.1074/jbc.M116.751933⟩. ⟨hal-01415795⟩

Share

Metrics

Record views

131