Linear and nonlinear Raman microspectroscopy: History, instrumentation, and applications

Hideaki Kano (1) , Hiroki Segawa , Philippe Leproux (2) , Vincent Couderc (2)

1 Université de Tsukuba = University of Tsukuba
2 XLIM-PHOT - Photonique Fibre et Sources Cohérentes

Through carrying out Raman spectroscopy under a microscope, we can observe the molecular distribution and dynamics of living cells in situ without staining or labeling. In general, Raman microscopy/microspectroscopy is classified into spontaneous Raman microscopy/microspectroscopy and nonlinear Raman microscopy/microspectroscopy. This review will first summarize the historical development of Raman microscopes, and will discuss in detail their instrumentation and applications.

Domaines: Optique / photonique

Hideaki Kano, Hiroki Segawa, Philippe Leproux, Vincent Couderc. Linear and nonlinear Raman microspectroscopy: History, instrumentation, and applications. Optical Review, 2014, 21 (6), pp. 752-761. ⟨10.1007/s10043-014-0123-9⟩. ⟨hal-01321424⟩