Extracting biological information from dna arrays : an unexpected link between arginine and methionine metabolism in bacillus subtilis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genome Biology Année : 2001

Extracting biological information from dna arrays : an unexpected link between arginine and methionine metabolism in bacillus subtilis

Résumé

Background: In global gene expression profiling experiments, variation in the expression of genes of interest can often be hidden by general noise. To determine how biologically significant variation can be distinguished under such conditions we have analyzed the differences in gene expression when Bacillus subtilis is grown either on methionine or on methylthioribose as sulfur source. Results: An unexpected link between arginine metabolism and sulfur metabolism was discovered, enabling us to identify a high-affinity arginine transport system encoded by the yqiXYZ genes. In addition, we tentatively identified a methionine/methionine sulfoxide transport system which is encoded by the operon ytmIJKLMhisP and is presumably used in the degradation of methionine sulfoxide to methane sulfonate for sulfur recycling. Experimental parameters resulting in systematic biases in gene expression were also uncovered. In particular, we found that the late competence operons comE, comF and comG were associated with subtle variations in growth conditions. Conclusions: Using variance analysis it is possible to distinguish between systematic biases and relevant gene-expression variation in transcriptome experiments. Co-variation of metabolic gene expression pathways was thus uncovered linking nitrogen and sulfur metabolism in B. subtilis.
Fichier principal
Vignette du fichier
art-2001-2-6-research0019-1.pdf (222.36 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01219561 , version 1 (30-11-2016)

Licence

Paternité

Identifiants

Citer

Agnieszka Sekowska, Stephane Robin, Jean-Jacques Daudin, Alain Hénaut, Antoine Danchin. Extracting biological information from dna arrays : an unexpected link between arginine and methionine metabolism in bacillus subtilis. Genome Biology, 2001, 2 (6), ⟨10.1186/gb-2001-2-6-research0019⟩. ⟨hal-01219561⟩
233 Consultations
58 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More