Vectorisation et délivrance ciblée de médicaments ou gènes inductibles par des nanoparticules sensibles à l'hyperthermie sous contrôle de l'IRM - NanoBioImaging
Abstract
In experimental models of glioblastoma and Multiple sclerosis, we took advantage of molecular alterations of the neurovascular cells to address specific targeting via immune cells (monocytes, microglia) and ligands (antibodies and newly screened peptide ligands). The microglia cells were manipulated ex vivo for the heat sensitive expression of a therapeutic gene and incorporated a heat sensitive magnetic contrast agent. The ligands were labelled to carry both a magnetic contrast agent integrated in a heat sensitive cargo and a fluorescent maker. Upon i.v. administration, the manipulated microglia cells and the molecular ligand constructs targeted respectively, in vivo the tumour sites and the inflammatory alterations of lesions of the central nervous system (CNS) under the in vivo control of MRI that was confirmed by immunohistopathology. The approach is developed to associate target specific therapy and biomarkers in CNS diseases.