Statistical study of blood cell populations by very wide-field bimodal phase/ fluorescence imaging

Isaure Le Cardinal de Kernier (1, 2) , Stéphanie Bressieux (3) , Nelly Rongeat (3) , Anais Ali-Cherif (3) , Sophie Morales (1) , Serge Monneret (2, 4) , Pierre Blandin (1)

1 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
2 FRESNEL - Institut FRESNEL
3 HORIBA ABX SAS - HORIBA Medical
4 MOSAIC - MOSAIC

Very wide-field of view imaging can provide statistical data on large cell populations in a single acquisition. It can be used as an alternative to standard fluorescence flow cytometry methods for applications in hematology. We will describe a multimodal imaging system combining brightfield, phase and fluorescence contrasts. Its greater simplicity and lower cost compared to flow cytometry make it suitable for Point-Of-Care applications. The system's resolution was characterized on calibrated beads and targets. We illustrate the potential of the single-shot imaging approach in hematology by showing classification of white blood cells morphologies.

Mots clés: optics; statistics; fluorescence; phase; in-line holography; microscopy

Domaines: Optique [physics.optics]

Isaure Le Cardinal de Kernier, Stéphanie Bressieux, Nelly Rongeat, Anais Ali-Cherif, Sophie Morales, et al.. Statistical study of blood cell populations by very wide-field bimodal phase/ fluorescence imaging. Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, Feb 2019, San Francisco, United States. pp.10, ⟨10.1117/12.2506531⟩. ⟨hal-02473883⟩