Fabrication and study of multidimensional scaffolds for cellular and tissue engineering

Abstract : The objective of this work is to develop a method of engineering multi-dimensional scaffolds for cell culture and tissue formation. We firstly applied a 3D printing technique to produce the designed frame in PEGDA and then filled the free-space of the frame with a gelatin gel. After freezing and drying, a hybrid 3D scaffold made of gelatin porous structures and PEDGA backbone was obtained, which supported culture and differentiation of neural progenitor cells. To more easily integrate into a microfluidic device, we also designed a 2D scaffold in form of a thin layer of honeycomb frame of PEGDA and self-assembled porous structure of PCL. Such a patch form scaffold could be used for cell culture and gene transfection, showing advantages over the conventional methods in terms of nutrients and soluble factors uptake. Finally, we fabricated a soft patch made of an elastic frame in PDMS and a monolayer of gelatin nanofibers to facilitate cardiac differentiation from human induced pluripotent stem cells. As expected, we achieved a cardiac generation with higher contraction strength and a higher beating homogeneity comparing to the conventional approaches. All together, we demonstrated the utility of hybrid scaffolds for micro-tissue engineering which could impact the future studies in the fields of tissue engineering, drug screening and regenerative medicine.
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Xiaolong Tu. Fabrication and study of multidimensional scaffolds for cellular and tissue engineering. Theoretical and/or physical chemistry. PSL Research University, 2017. English. ⟨NNT : 2017PSLEE045⟩. ⟨tel-01816990⟩

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