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In vivo imaging of vessel diameter, size, and density: a comparative study between MRI and histology.: Vessel diameter, size and density: MRI vs histology

Benjamin Lemasson 1 Samuel Valable 2 Régine Farion 3 Alexandre Krainik 4 Chantal Rémy 1 Emmanuel Barbier 1, *
* Corresponding author
2 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
CI-NAPS - UMR 6232 - Centre-Imagerie, Neurosciences, et Application aux Pathologies, GIN - Grenoble Institut des Neurosciences
4 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
GIN - Grenoble Institut des Neurosciences, Service de neuroradiologie [Grenoble]
Abstract : The aim of this study was to compare magnetic resonance imaging (MRI) and histological estimates of the mean vessel diameter (mVD), the vessel density (Density), and the vessel size index (VSI) obtained in the same tumor-bearing animals. Twenty-seven rats bearing intracranial glioma (C6 or RG2) were imaged by MRI. Changes in transverse relaxations (ΔR 2* and R(2)) were induced by the injection of an iron-based contrast agent and were mapped using a multi gradient-echo spin-echo sequence. Then, brain vascular network was studied ex vivo by histology. Three regions of interest were drawn in apparently normal tissue (neocortex and striatum) and in the tumor. In vivo mVD(MRI), Density(MRI), and VSI(MRI) were measured; ex vivo, mVD(histo), Density(histo), and VSI(histo) were quantified on the same animals. MRI and histology measurements differed by -15 to 26%. A positive correlation was found between MRI and histology for mVD, Density, and VSI counterparts (R(2) = 0.62, 0.50, 0.73, respectively; P < 0.001 in all cases). This study indicates that MRI and histology yields well correlated the estimates of mVD, Density, and VSI. VSI is the closest MRI estimate to histology. As Density and mVD or VSI provide complementary information, it is worth computing them to characterize angiogenesis beyond blood volume fraction.
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Submitted on : Thursday, September 12, 2013 - 10:29:17 AM
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Benjamin Lemasson, Samuel Valable, Régine Farion, Alexandre Krainik, Chantal Rémy, et al.. In vivo imaging of vessel diameter, size, and density: a comparative study between MRI and histology.: Vessel diameter, size and density: MRI vs histology. Magnetic Resonance in Medicine, Wiley, 2013, 69 (1), pp.18-26. ⟨10.1002/mrm.24218⟩. ⟨inserm-00861168⟩

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