NMR-based metabolomic analysis of the physiological role of the electron-bifurcating FeFehydrogenase Hnd in Solidesulfovibrio fructosivorans under pyruvate fermentation

Natalie Payne (1, 2) , Arlette Kpebe (1) , Chloé Guendon (1) , Carole Baffert (1) , Matthieu Maillot (3) , Typhaine Haurogné (3) , Fabrice Tranchida (2) , Myriam Brugna (1) , Laetitia Shintu (2)

1 BIP - Bioénergétique et Ingénierie des Protéines
2 ISM2 - Institut des Sciences Moléculaires de Marseille
3 MS Nutrition [Marseille, France]

Solidesulfovibrio fructosivorans (formely Desulfovibrio fructosovorans), an anaerobic sulfate-reducing bacterium, possesses six gene clusters encoding six hydrogenases catalyzing the reversible oxidation of hydrogen gas (H2) into protons and electrons. One of these, named Hnd, was demonstrated to be an electronbifurcating hydrogenase Hnd (Kpebe et al., 2018). It couples the exergonic reduction of NAD + to the endergonic reduction of a ferredoxin with electrons derived from H2 and whose function has been recently shown to be involved in ethanol production under pyruvate fermentation (Payne 2022). To understand further the physiological role of Hnd in S. fructosivorans, we compared the mutant deleted of part of the hnd gene with the wild-type strain grown on pyruvate without sulfate using NMR-based metabolomics. Our results confirm that Hnd is profoundly involved in ethanol metabolism, but also indirectly intervenes in global carbon metabolism and additional metabolic processes such as the biosynthesis of branched-chain amino acids. We also highlight the metabolic reprogramming induced by the deletion of hndD that leads to the upregulation of several NADP-dependent pathways. Highlights • Metabolomics characterized pathways of pyruvate fermentation in S. fructosivorans • Hnd is involved in ethanol metabolism and branched-chain amino acid biosynthesis. • HndD deletion leads to the upregulation of NADP-dependent pathways.

Mots clés: Metabolomics; electron-bifurcating hydrogenase; ethanol metabolism; NADP-dependent pathways; Desulfovibrio fructosovorans; metabolomics electron-bifurcating hydrogenase ethanol metabolism NADP-dependent pathways Desulfovibrio; metabolomics; Desulfovibrio

Domaines: Bactériologie; Biochimie [q-bio.BM]

Natalie Payne, Arlette Kpebe, Chloé Guendon, Carole Baffert, Matthieu Maillot, et al.. NMR-based metabolomic analysis of the physiological role of the electron-bifurcating FeFehydrogenase Hnd in Solidesulfovibrio fructosivorans under pyruvate fermentation. Microbiological Research, 2022, 268, pp.127279. ⟨10.1016/j.micres.2022.127279⟩. ⟨hal-04382053⟩