A comparative multi-level toxicity assessment of carbon-based Gd-free dots and Gd-doped nanohybrids from coffee waste: hematology, biochemistry, histopathology and neurobiology study

Halyna Kuznietsova (1) , Natalia Dziubenko (1) , Konstantin Paliienko (2) , Natalia Pozdnyakova (2) , Natalia Krisanova (2) , Artem Pastukhov (2) , Tetiana Lysenko (2) , Marina Dudarenko (2) , Valeriy Skryshevsky (3) , Vladimir Lysenko (4) , Tatiana Borisova (2)

1 Corporation Science Park, Taras Shevchenko National University of Kyiv
2 Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine
3 Taras Shevchenko National University of Kyiv
4 iLM - FENNEC - iLM - Formation, élaboration de nanomatériaux et cristaux

Here, a comparative toxicity assessment of precursor carbon dots from coffee waste (cofCDs) obtained using green chemistry principles and Gd-doped nanohybrids (cofNHs) was performed using hematological, biochemical, histopathological assays in vivo (CD1 mice, intraperitoneal administration, 14 days), and neurochemical approach in vitro (rat cortex nerve terminals, synaptosomes). Serum biochemistry data revealed similar changes in cofCDs and cofNHs-treated groups, i.e. no changes in liver enzymes' activities and creatinine, but decreased urea and total protein values. Hematology data demonstrated increased lymphocytes and concomitantly decreased granulocytes in both groups, which could evidence inflammatory processes in the organism and was confirmed by liver histopathology; decreased red blood cell-associated parameters and platelet count, and increased mean platelet volume, which might indicate concerns with platelet maturation and was confirmed by spleen histopathology. So, relative safety of both cofCDs and cofNHs for kidney, liver and spleen was shown, whereas there were concerns about platelet maturation and erythropoiesis. In acute neurotoxicity study, cofCDs and cofNHs (0.01 mg/ml) did not affect the extracellular level of L-[ 14 C]glutamate and [ 3 H]GABA in nerve terminal preparations. Therefore, cofNHs demonstrated minimal changes in serum biochemistry and hematology assays, had no acute neurotoxicity signs, and can be considered as perspective biocompatible non-toxic theragnostic agent.

Mots clés: PLASMA-MEMBRANE; GLUTAMATE; NANOPARTICLES; TRANSPORT; PLANETARY; PROTON; LEVEL; BEANS; SHELL; MICE

Domaines: Physique [physics]

Halyna Kuznietsova, Natalia Dziubenko, Konstantin Paliienko, Natalia Pozdnyakova, Natalia Krisanova, et al.. A comparative multi-level toxicity assessment of carbon-based Gd-free dots and Gd-doped nanohybrids from coffee waste: hematology, biochemistry, histopathology and neurobiology study. Scientific Reports, 2023, 13 (1), pp.9306. ⟨10.1038/s41598-023-36496-4⟩. ⟨hal-04209145⟩