Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebrafish

Christine Thisse (1) , Agnès Degrave , Gregory V. Kryukov , Vadim N. Gladyshev , Sophie Obrecht-Pflumio , Alain Krol (2, 3) , Bernard Thisse (1) , Alain Lescure (2, 3)

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
2 ARN - Architecture et réactivité de l'ARN
3 ARN - Architecture et Réactivité de l'ARN

Selenium is important for embryogenesis in vertebrates but little is known about the expression patterns and biological functions of most selenoprotein genes. Taking advantage of the zebrafish model, systematic analysis of selenoprotein gene expression was performed by in situ hybridization on whole-mount embryos at different developmental stages. Twenty-one selenoprotein mRNAs were analyzed and all of them exhibited expression patterns restricted to specific tissues. Moreover, we demonstrated that highly similar selenoprotein paralogs were expressed within distinct territories. Therefore, tissue- and development-specific expression patterns provided new information for selenoproteins of unknown function.

Domaines: Biochimie, Biologie Moléculaire

Christine Thisse, Agnès Degrave, Gregory V. Kryukov, Vadim N. Gladyshev, Sophie Obrecht-Pflumio, et al.. Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebrafish. Gene expression patterns: GEP, 2003, 3 (4), pp.525-532. ⟨10.1016/s1567-133x(03)00054-1⟩. ⟨hal-04151459⟩