Identification of 1088 new transposon insertions of Caenorhabditis elegans: a pilot study toward large-scale screens

Edwige Martin (1) , Hélène Laloux (1) , Gaëlle Couette (1) , Thierry Alvarez (1) , Catherine Bessou (1) , Oliver Hauser (1) , Satis Sookhareea (1) , Michel Labouesse (1) , Laurent Ségalat (1)

1 CGPhiMC - Centre de génétique et de physiologie moléculaire et cellulaire

We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.

Domaines: Génétique

Edwige Martin, Hélène Laloux, Gaëlle Couette, Thierry Alvarez, Catherine Bessou, et al.. Identification of 1088 new transposon insertions of Caenorhabditis elegans: a pilot study toward large-scale screens. Genetics, 2002, 162 (1), pp.521-524. ⟨10.1093/genetics/162.1.521⟩. ⟨hal-04151455⟩