NSD1 is essential for early post-implantation development and has a catalytically active SET domain

Geetha Vani Rayasam (1) , Olivia Wendling (2) , Pierre-Olivier Angrand , Manuel Mark (2) , Karen Niederreither (3) , Luyan Song (2) , Thierry Lerouge (2) , Gordon L. Hager (1) , Pierre Chambon (2) , Regine Losson (2)

1 NIH - National Institutes of Health [Bethesda, MD, USA]
2 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
3 BCM - Baylor College of Medicine

The nuclear receptor-binding SET domain-containing protein (NSD1) belongs to an emerging family of proteins, which have all been implicated in human malignancy. To gain insight into the biological functions of NSD1, we have generated NSD1-deficient mice by gene disruption. Homozygous mutant NSD1 embryos, which initiate mesoderm formation, display a high incidence of apoptosis and fail to complete gastrulation, indicating that NSD1 is a developmental regulatory protein that exerts function(s) essential for early post-implantation development. We have also examined the enzymatic potential of NSD1 and found that its SET domain possesses intrinsic histone methyltransferase activity with specificity for Lys36 of histone H3 (H3-K36) and Lys20 of histone H4 (H4-K20).

Domaines: Biochimie, Biologie Moléculaire

Geetha Vani Rayasam, Olivia Wendling, Pierre-Olivier Angrand, Manuel Mark, Karen Niederreither, et al.. NSD1 is essential for early post-implantation development and has a catalytically active SET domain. EMBO Journal, 2003, 22 (12), pp.3153-3163. ⟨10.1093/emboj/cdg288⟩. ⟨hal-04145127⟩