Murine spermatogonial stem cells: targeted transgene expression and purification in an active state

Galicia Giuili (1) , Andrea Tomljenovic (1) , Nathalie Labrecque (1) , Mustapha Oulad-Abdelghani (2) , Minoo Rassoulzadegan (1) , François Cuzin (1)

1 Génétique moléculaire de la différenciation et du développement
2 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

A 400 bp fragment of the spermatogonia-specific Stra8 locus was sufficient to direct gene expression to the germinal stem cells in transgenic mice. A fractionation procedure was devised, based on immunomagnetic sorting of cells in which the promoter drives the expression of a surface functionally neutral protein tag. The purified cells expressed the known molecular markers of spermatogonia Rbm, cyclin A2 and EP-Cam, and the beta1- and alpha6-integrins characteristic of the stem cell fraction. A 700-fold enrichment in stem cells was determined by the ability of the purified fractions to re-establish spermatogenesis in germ cell-depleted recipient testes.

Domaines: Biochimie, Biologie Moléculaire

Galicia Giuili, Andrea Tomljenovic, Nathalie Labrecque, Mustapha Oulad-Abdelghani, Minoo Rassoulzadegan, et al.. Murine spermatogonial stem cells: targeted transgene expression and purification in an active state. EMBO Reports, 2002, 3 (8), pp.753-759. ⟨10.1093/embo-reports/kvf149⟩. ⟨hal-04142448⟩