The NIZP1 KRAB and C2HR domains cross-talk for transcriptional regulation

Regine Losson (1, 1) , Anders L. Nielsen

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

The NSD1 histone methyltransferase is involved in the outgrowth disorders Sotos and Weaver syndromes and childhood acute myeloid leukemia. NSD1 is a bona fida transcriptional co-repressor for Nizp1 which is a protein including SCAN, KRAB, C2HR and zinc-finger domains. In this study the Nizp1 KRAB-domain was identified to possess an intrinsic transcriptional activation capacity suppressed in cis by the presence of the C2HR domain. Oppositely, the KRAB-domain supported C2HR domain mediated transcriptional repression. The presence of the KRAB-domain resulted in increased NSD1 co-repressor association with the C2HR domain. This study shows a new function of the KRAB-domain, C2HR-domain, and the associated factors to confer Nizp1 mediated transcriptional regulation.

Domaines: Biochimie, Biologie Moléculaire

Regine Losson, Anders L. Nielsen. The NIZP1 KRAB and C2HR domains cross-talk for transcriptional regulation. BBA - Biochimica et Biophysica Acta, 2010, 1799 (5-6), pp.463-468. ⟨10.1016/j.bbagrm.2010.02.003⟩. ⟨hal-04065523⟩