Rfx6 Maintains the Functional Identity of Adult Pancreatic β Cells

Julie Piccand (1) , Perrine Strasser (1) , David J. Hodson (2) , Aline Meunier (1) , Tao Ye (1) , Céline Keime (1) , Marie-Christine Birling (1) , Guy A. Rutter (2) , Gérard Gradwohl (1)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 Imperial College London

Increasing evidence suggests that loss of β cell characteristics may cause insulin secretory deficiency in diabetes, but the underlying mechanisms remain unclear. Here, we show that Rfx6, whose mutation leads to neonatal diabetes in humans, is essential to maintain key features of functionally mature β cells in mice. Rfx6 loss in adult β cells leads to glucose intolerance, impaired β cell glucose sensing, and defective insulin secretion. This is associated with reduced expression of core components of the insulin secretion pathway, including glucokinase, the Abcc8/SUR1 subunit of KATP channels and voltage-gated Ca2+ channels, which are direct targets of Rfx6. Moreover, Rfx6 contributes to the silencing of the vast majority of “disallowed” genes, a group usually specifically repressed in adult β cells, and thus to the maintenance of β cell maturity. These findings raise the possibility that changes in Rfx6 expression or activity may contribute to β cell failure in humans

Domaines: Organisation et fonctions cellulaires [q-bio.SC]; Endocrinologie et métabolisme

Julie Piccand, Perrine Strasser, David J. Hodson, Aline Meunier, Tao Ye, et al.. Rfx6 Maintains the Functional Identity of Adult Pancreatic β Cells. Cell Reports, 2014, 9 (6), pp.2219-2232. ⟨hal-04004335⟩