Crystallization of threonyl-tRNA synthetase from Thermus thermophilus and preliminary crystallographic data

Vincent Cura (1, 2) , Daniel Kern (3, 4) , André Mitschler (1, 2) , Dino Moras (1, 2)

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
2 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
3 ARN - Architecture et Réactivité de l'ARN
4 ARN - Architecture et réactivité de l'ARN

Threonyl-tRNA synthetase from Thermus thermophilus (ttTRS) has been overproduced in Escherichia coli, purified and crystallized in solutions containing ammonium sulfate and glycerol. The crystals grew in the orthorhombic space group C2221 with unit cell dimensions a = 119.5 Å, b = 120.0 Å, c = 317.5 Å. The asymmetric unit is constituted of two monomers and the crystals contain 66% solvent. This paper reports the first crystals of ttTRS and preliminary crystallographic results since the presumed crystals of ttTRS described in a previous paper [1] were crystals of aspartyl-tRNA synthetase [2].

Mots clés: tRNA

Domaines: Biochimie, Biologie Moléculaire

Vincent Cura, Daniel Kern, André Mitschler, Dino Moras. Crystallization of threonyl-tRNA synthetase from Thermus thermophilus and preliminary crystallographic data. FEBS Letters, 1995, 374 (1), pp.110-112. ⟨10.1016/0014-5793(95)01089-W⟩. ⟨hal-03831428⟩