Container material dictates stability of bacteriophage suspensions: Light scattering and infectivity measurements reveal mechanisms of infectious titre decay

Larry O'Connell (1, 2) , Yoann Roupioz (1) , Pierre R Marcoux (2)

1 CREAB - Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques
2 DTBS - Département Microtechnologies pour la Biologie et la Santé

Aims: To measure the infectious titer (IT) decay rate for various bacteriophages as a function of storage container material. Additionally, parallel light scattering and infectious titer measurements reveal distinct mechanisms for IT loss, depending on phage. Methods and Results: Suspensions of bacteriophages 44AHJD, P68, and gh-1 were stored in various labware. IT of each suspension was repeatedly measured over the course of two weeks. Large variability in IT decay was observed, with >4 log10 loss in glass and low-binding polypropylene.

Mots clés: Bacteriophage; colloidal stability; aggregation; virus adsorption; nanoparticle tracking analysis; infectious titer; phage stability; plaque counting

Domaines: Biotechnologies; Virologie

Larry O'Connell, Yoann Roupioz, Pierre R Marcoux. Container material dictates stability of bacteriophage suspensions: Light scattering and infectivity measurements reveal mechanisms of infectious titre decay. Journal of Applied Microbiology, 2022, 133 (2), pp.529-543. ⟨10.1111/jam.15581⟩. ⟨hal-03720069⟩