Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins

B Jester , Robert Drillien (1, 2) , Marc Ruff (2, 1) , Catherine Florentz (3, 4)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 IGBMC - Institut de génétique et biologie moléculaire et cellulaire
3 ARN - Architecture et Réactivité de l'ARN
4 ARN - Architecture et réactivité de l'ARN

Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.

Mots clés: tRNA; Recombinant; Vaccinia; T7; Transfection; Infection; MVA

Domaines: Génétique

B Jester, Robert Drillien, Marc Ruff, Catherine Florentz. Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins. Journal of biotechnology, 2011, 156 (3), pp.211-213. ⟨10.1016/j.jbiotec.2011.09.013⟩. ⟨hal-03702227⟩