Understanding Chromosome Structure During Early Mouse Development by a Single-Cell Hi-C Analysis

Noémie Ranisavljevic (1, 2, 3) , Maud Borensztein (4, 3) , Katia Ancelin (3)

1 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
2 UM - Université de Montpellier
3 Génétique et Biologie du Développement
4 IGMM - Institut de Génétique Moléculaire de Montpellier

Over the past two decades, the development of chromosome conformation capture technologies has allowed to intensively probe the properties of genome folding in various cell types. High-throughput versions of these C-based assays (named Hi-C) have released the mapping of 3D chromosome folding for the entire genomes. Applied to mammalian preimplantation embryos, it has revealed a unique chromosome organization after fertilization when a new individual is being formed. However, the questions of whether specific structures could arise depending on their parental origins or of their transcriptional status remain open. Our method chapter is dedicated to the technical description on how applying scHi-C to mouse embryos at different stages of preimplantation development. This approach capitalized with the limited amount of material available at these developmental stages. It also provides new research avenues, such as the study of mutant embryos for further functional studies.

Mots clés: 3D genome organization; Chromosome conformation capture; Mouse preimplantation embryo; Single-cell Hi-C

Domaines: Sciences du Vivant [q-bio]

Noémie Ranisavljevic, Maud Borensztein, Katia Ancelin. Understanding Chromosome Structure During Early Mouse Development by a Single-Cell Hi-C Analysis. Epigenetic Reprogramming During Mouse Embryogenesis, 2214, Springer US, pp.283-293, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0958-3_19⟩. ⟨hal-03652607⟩