HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Fast and novel botanical exploration of a 320-km transect in eastern Amazonia using DNA barcoding

Abstract : We explored a 320-km transect in the Tumucumaque mountain range along the border between southern French Guiana and Brazil, sampling all trees and lianas with DBH ≥ 10 cm in seven 25 x 25-m plots installed near seven boundary milestones. We isolated DNA from cambium tissue and sequenced two DNA barcodes (rbcLa and matK) to aid in species identification. We also collected fertile herbarium specimens from other species (trees/shrubs/herbs) inside and outside the plots. The selected DNA barcodes were useful at the family level but failed to identify specimens at the species level. Based on DNA barcoding identification, the most abundant families in the plots were Burseraceae, Fabaceae, Meliaceae, Moraceae, Myristicaceae and Sapotaceae. One third of the images of sampled plants posted on the iNaturalist website were identified by the community to species level. New approaches, including the sequencing of the ITS region and fast evolving DNA plastid regions, remain to be tested for their utility in the identification of specimens at lower taxonomic levels in floristic inventories in the Amazon region.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03639836
Contributor : François-Michel Le Tourneau Connect in order to contact the contributor
Submitted on : Wednesday, April 13, 2022 - 10:03:07 AM
Last modification on : Friday, May 13, 2022 - 3:40:17 AM

File

CPqShnvd4xvp9Nx5Y54Kkgp.pdf
Publisher files allowed on an open archive

Identifiers

Citation

William Milliken, Guillaume Odonne, Julien Engel, François-Michel Le Tourneau, Uxue Suescun, et al.. Fast and novel botanical exploration of a 320-km transect in eastern Amazonia using DNA barcoding. Acta Amazonica, Instituto Nacional de Pesquisas da Amazônia, 2022, 52 (1), pp.29-37. ⟨10.1590/1809-4392202101413⟩. ⟨hal-03639836⟩

Share

Metrics

Record views

60

Files downloads

3