A Comparative Study of Maize and Miscanthus Regarding Cell-Wall Composition and Stem Anatomy for Conversion into Bioethanol and Polymer Composites

M. Brancourt-Hulmel (1, 2) , S. Arnoult (3) , L. Cézard (4) , F El Hage (4) , E. Gineau (4) , J. Girones (5) , Y. Griveau (4) , M.-P. Jacquemont (4) , S. Jaffuel (6, 7) , E. Mignot (3) , G. Mouille (4) , C. Lapierre (4) , F. Legée (4) , V. Méchin (4) , Patrick Navard (5) , L. T. T. Vo (5) , M. Reymond (4)

1 BioEcoAgro - UMR transfrontalière INRAE 1158
2 BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
3 GCIE - Unité Expérimentale Grandes Cultures Innovation Environnement - Picardie
4 IJPB - Institut Jean-Pierre Bourgin
5 CEMEF - Centre de Mise en Forme des Matériaux
6 Cirad-BIOS - Département Systèmes Biologiques
7 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales

Due to an increasing demand for environmentally sustainable products, miscanthus and maize stover represent interesting lignocellulosic resources for conversion into biofuels and biomaterials. The overall purpose was to compare miscanthus and maize regarding cell-wall composition and stem anatomy for conversion into bioethanol and polymer composites using partial least squares regressions. For each of the two crops, six contrasted genotypes were cultivated in complete block design, and harvested. Internodes below the main cob for maize, and on the first aboveground internode for miscanthus, were analyzed for biochemistry and anatomy. Their digestibility was predicted using crop-specific near infrared calibrations, and the mechanical properties were evaluated in stem-based composites. On average, the internode cross-section of miscanthus anatomy was characterized by a thick rind (26.2 %) and few but dense pith-bundles (3.5 nb/mm²), while cell-wall constituted 95.2 % of the dry matter with high lignin (243.2 mg/g) and cellulose concentrations (439.7 mg/g). Maize internode-anatomy showed large cross-sections (397.5 mm²), pith with the presence of numerous bundles and non-lignified-pith fractions (22.3 % of the section). Its cellwall biochemistry displayed high concentrations of hemicelluloses, galactose, arabinose, xylose and ferulic acid. Cell-wall, lignin and cellulose concentrations were positively correlated with rind-fraction and pith-bundle-density, which explained strong mechanical properties as shown in miscanthus. Hemicelluloses, galactose, arabinose and ferulic acid concentrations were positively correlated with pith fraction and stem cross-section, revealing high digestibility as shown in maize. This underlines interesting traits for further comparative genetic studies, as maize represents a good model for digestibility and miscanthus for composites.

Mots clés: biomaterials; polysaccharides; enzymatic hydrolysis; biofuels; Energy crop; lignocellulosic biomass

Domaines: Matériaux; Polymères

M. Brancourt-Hulmel, S. Arnoult, L. Cézard, F El Hage, E. Gineau, et al.. A Comparative Study of Maize and Miscanthus Regarding Cell-Wall Composition and Stem Anatomy for Conversion into Bioethanol and Polymer Composites. BioEnergy Research, 2022, 15, pp.777-791. ⟨10.1007/s12155-020-10239-z⟩. ⟨hal-03517627⟩