Molecular recognition of the closely related Fraxinus excelsior and F. oxyphylla (Oleaceae) by RAPD markers

Sylvain Jeandroz , Nathalie Frascaria-Lacoste (1) , Jean Bousquet

1 ESE - Ecologie Systématique et Evolution

RAPD markers were designed to identify two closely related European ash species, Fraxinus excelsior and F. oxyphylla and their putative hybrids. From the use of 210 primers, nine species-specific fragments were identified. These markers were found to be monomorphic or nearly monomorphic in either F. excelsior or F. oxyphylla or were present in frequencies which differed greatly between the two species. Southern blot analyses were conducted to verify the homology of the diagnostic fragments. Fingerprints obtained for 16 putative hybrids from the sympatric zone showed recombination of the species-specific markers, indicating a potentially high level of introgressive hybridization

Domaines: Sciences du Vivant [q-bio]

Sylvain Jeandroz, Nathalie Frascaria-Lacoste, Jean Bousquet. Molecular recognition of the closely related Fraxinus excelsior and F. oxyphylla (Oleaceae) by RAPD markers. International Journal of Forest Genetics, 1996, 3 (4), pp.237-242. ⟨hal-03494297⟩