Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

Ali Atoui , Huy Phong Dao , Florence Mathieu (1) , Ahmed Lebrihi (1)

1 LGC - Laboratoire de Génie Chimique

The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.

Mots clés: Aspergillus carbonarius; Aspergillus ochraceus; Mycotoxins; Ochratoxin A; Polyketide synthase

Domaines: Génie chimique

Ali Atoui, Huy Phong Dao, Florence Mathieu, Ahmed Lebrihi. Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. Molecular Nutrition & Food Research, 2006, 5 (6), pp.488-493. ⟨10.1002/mnfr.200500165⟩. ⟨hal-03482063⟩