A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation

Kinga Kamieniarz (1) , Annalisa Izzo (1, 2) , Miroslav Dundr (3) , Philipp Tropberger (1) , Luka Ozretić (4) , Jutta Kirfel (5) , Elisabeth Scheer (2) , Philippe Tropel (2) , Jacek Wiśniewski , Laszlo Tora (2) , Stephane Viville (2) , Reinhard Buettner (4) , Robert Schneider (2, 1, 6)

1 MPI-IE - Max Planck Institute of Immunobiology and Epigenetics
2 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
3 Chicago Medical School [Rosalind Franklin University]
4 University of Cologne
5 Universität Bonn = University of Bonn
6 Albert-Ludwigs-Universität Freiburg

The linker histone H1 is a key player in chromatin organization, yet our understanding of the regulation of H1 functions by post-translational modifications is very limited. We provide here the first functional characterization of H1 acetylation. We show that H1.4K34 acetylation (H1.4K34ac) is mediated by GCN5 and is preferentially enriched at promoters of active genes, where it stimulates transcription by increasing H1 mobility and recruiting a general transcription factor. H1.4K34ac is dynamic during spermatogenesis and marks undifferentiated cells such as induced pluripotent stem (iPS) cells and testicular germ cell tumors. We propose a model for H1.4K34ac as a novel regulator of chromatin function with a dual role in transcriptional activation.

Domaines: Sciences du Vivant [q-bio]

Kinga Kamieniarz, Annalisa Izzo, Miroslav Dundr, Philipp Tropberger, Luka Ozretić, et al.. A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation. Genes and Development, 2012, 26 (8), pp.797-802. ⟨10.1101/gad.182014.111⟩. ⟨hal-03464633⟩