AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1 -KO mouse model deficits of fragile X syndrome

Karima Habbas , Oktay Cakil , Boglarka Zambo , Ricardos Tabet , Fabrice Riet , Doulaye Dembele , Jean-Louis Mandel , Michaël Hocquemiller , Ralph Laufer , Françoise Piguet , Hervé Moine (1)

1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire

Fragile X syndrome (FXS) is the most frequent form of familial intellectual disability. It results from the lack of the RNA binding protein FMRP and is associated with the overactivation of signaling pathways downstream of mGluRI receptors and upstream of mRNA translation. We previously found that diacylglycerol kinase kappa (DGKk) is a main mRNA target of FMRP in cortical neurons. Here we show that diacylglycerol kinase kappa (DGKk), when modified as to become FMRP-independent and delivered into the brain of adolescent mice using adeno-associated viral vectors, corrects brain diacylglycerol and phosphatidic acid homeostasis and the main phenotypic behaviors of the Fmr1-KO mouse model of FXS. Thus, DGKk appears as a key triggering factor of FXS pathomechanism while providing a possible means of intervention for FXS gene therapy.

Domaines: Sciences du Vivant [q-bio]

Karima Habbas, Oktay Cakil, Boglarka Zambo, Ricardos Tabet, Fabrice Riet, et al.. AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1 -KO mouse model deficits of fragile X syndrome. 2021. ⟨hal-03450738⟩