Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy

Dari Da (1) , Ruth Mccabe , Bernard Somé , Pedro Esperança , Katarzyna Sala , Josua Blight , Andrew Blagborough , Floyd Dowell , Serge Yerbanga , Thierry Lefèvre (2) , Karine Mouline (3) , Roch Dabiré , Thomas Churcher

1 IRSS - Institut de Recherche en Sciences de la Santé
2 MIVEGEC-TRIAD - Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes
3 MIVEGEC - Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle

There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.

Domaines: Sciences du Vivant [q-bio]

Dari Da, Ruth Mccabe, Bernard Somé, Pedro Esperança, Katarzyna Sala, et al.. Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy. Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-89715-1⟩. ⟨hal-03450542⟩