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3D Image super-resolution by fluorophore fluctuations and ma-tirf microscopy reconstruction (3D-col0rme)

Vasiliki Stergiopoulou 1 Luca Calatroni 1 Sébastien Schaub 2 Laure Blanc-Féraud 1 
1 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D-Covariance-based ℓ 0 super-Resolution Microscopy with intensity Estimation) improves both lateral and axial resolution by combining sparsity-based modelling for precise molecule localisation and intensity estimation in the lateral plane with a 3D reconstruction procedure in the axial one using Multi-Angle TIRF (MA-TIRF). Differently from state-of-the-art approaches, 3D-COL0RME does not require the use of special equipment as it can be used with standard fluorophores. We validate 3D-COL0RME on simulated MA-TIRF blinking-type data and on challenging real MA-TIRF acquisitions, showing significant resolution improvements.
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https://hal.archives-ouvertes.fr/hal-03412778
Contributor : Luca Calatroni Connect in order to contact the contributor
Submitted on : Thursday, March 31, 2022 - 7:05:20 PM
Last modification on : Thursday, August 4, 2022 - 4:55:04 PM

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  • HAL Id : hal-03412778, version 2

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Vasiliki Stergiopoulou, Luca Calatroni, Sébastien Schaub, Laure Blanc-Féraud. 3D Image super-resolution by fluorophore fluctuations and ma-tirf microscopy reconstruction (3D-col0rme). 2022. ⟨hal-03412778v2⟩

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