Monitoring eIF4E-Dependent Nuclear 3′ End mRNA Cleavage

Mildred Delaleau (1)

1 CBM - Centre de biophysique moléculaire

In eukaryotes, the maturation of the 3' ends of most transcripts involves cleavage and polyadenylation steps in the nucleus. While I was working in the group of Katherine Borden at the University of Montréal, we reported that the eukaryotic translation initiation factor 4E (eIF4E) promotes the 3' end cleavage of specific RNAs. Here, I describe how we monitored this specific maturation pathway using subcellular fractionation, quantitative RT-PCR, and an in vitro cleavage assay with the nuclear fraction.

Domaines: Sciences du Vivant [q-bio]

Mildred Delaleau. Monitoring eIF4E-Dependent Nuclear 3′ End mRNA Cleavage. Marc Boudvillain. RNA Remodeling Proteins: Methods and Protocols, 2209, Springer; Humana, pp.347-361, 2021, Methods in Molecular Biology, 978-1-0716-0934-7. ⟨10.1007/978-1-0716-0935-4_22⟩. ⟨hal-03335717⟩