Large-scale phenotyping of 1,000 fungal strains for the degradation of non-natural, industrial compounds

David Navarro (1) , Delphine Chaduli (1) , Sabine Taussac (1) , Laurence Lesage-Meessen (1) , Sacha Grisel (1) , Mireille Haon (1) , Philippe Callac (2) , Régis Courtecuisse (3) , Cony Decock (4) , Joëlle Dupont (5) , F. Richard-Forget (2) , Jacques Fournier , Jacques Guinberteau (2) , Christian Lechat (6) , Pierre-Arthur Moreau (3) , Laetitia Pinson-Gadais (2) , Bernard Rivoire (7) , Lucile Sage (8) , Stéphane Welti (3) , Marie-Noëlle Rosso (1) , Jean-Guy Berrin (1) , Bastien Bissaro (1) , Anne Favel (1)

1 BBF - Biodiversité et Biotechnologie Fongiques
2 MycSA - Unité de recherche Mycologie et Sécurité des Aliments
3 LGCgE - Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515
4 Mycothèque de l'Université catholique de Louvain (BCCMTM/MUCL)
5 ISYEB - Institut de Systématique, Evolution, Biodiversité
6 ASCOfrance
7 Société Linnéenne de Lyon
8 LECA - Laboratoire d'Ecologie Alpine

Fungal biotechnology is set to play a keystone role in the emerging bioeconomy, notably to address pollution issues arising from human activities. Because they preserve biological diversity, Biological Resource Centres are considered as critical infrastructures to support the development of biotechnological solutions. Here, we report the first large-scale phenotyping of more than 1,000 fungal strains with evaluation of their growth and degradation potential towards five industrial, human-designed and recalcitrant compounds, including two synthetic dyes, two lignocellulose-derived compounds and a synthetic plastic polymer. We draw a functional map over the phylogenetic diversity of Basidiomycota and Ascomycota , to guide the selection of fungal taxa to be tested for dedicated biotechnological applications. We evidence a functional diversity at all taxonomic ranks, including between strains of a same species. Beyond demonstrating the tremendous potential of filamentous fungi, our results pave the avenue for further functional exploration to solve the ever-growing issue of ecosystems pollution.

Domaines: Mycologie; Systématique, phylogénie et taxonomie

David Navarro, Delphine Chaduli, Sabine Taussac, Laurence Lesage-Meessen, Sacha Grisel, et al.. Large-scale phenotyping of 1,000 fungal strains for the degradation of non-natural, industrial compounds. Communications Biology, 2021, 4 (1), pp.871. ⟨10.1038/s42003-021-02401-w⟩. ⟨hal-03313721⟩