Establishment of actinorhizal symbiosis in response to ethylene, salicylic acid, and jasmonate

M. Ngom (1, 2, 3) , M. Cissoko (1, 2) , K. Gray (1) , Valérie Hocher (4) , Laurent Laplaze (5) , M.O. Sy (1, 3) , Sergio Svistoonoff (4, 1, 3) , Antony Champion (5)

1 Laboratoire Mixte International Adaptation des Plantes et microorganismes associés aux Stress Environnementaux
2 Institut Sénégalais de Recherches Agricoles, Institut de Recherche pour le Développement, Laboratoire Commun de Microbiologie
3 Faculté des Sciences et Techniques, Département de Biologie Végétale, Laboratoire Campus de Biotechnologies Végétales
4 UMR LSTM - Laboratoire des symbioses tropicales et méditerranéennes
5 UMR DIADE - Diversité, adaptation, développement des plantes

Phytohormones play a crucial role in regulating plant developmental processes. Among them, ethylene and jasmonate are known to be involved in plant defense responses to a wide range of biotic stresses as their levels increase with pathogen infection. In addition, these two phytohormones have been shown to inhibit plant nodulation in legumes. Here, exogenous salicylic acid (SA), jasmonate acid (JA), and ethephon (ET) were applied to the root system of Casuarina glauca plants before Frankia inoculation, in order to analyze their effects on the establishment of actinorhizal symbiosis. This protocol further describes how to identify putative ortholog genes involved in ethylene and jasmonate biosynthesis and/or signaling pathways in plant, using the Arabidopsis Information Resource (TAIR), Legume Information System (LIS), and Genevestigator databases. The expression of these genes in response to the bacterium Frankia was analyzed using the gene atlas for Casuarina-Frankia symbiosis (SESAM web site).

Domaines: Sciences du Vivant [q-bio]; Biologie végétale; Sciences de l'environnement

M. Ngom, M. Cissoko, K. Gray, Valérie Hocher, Laurent Laplaze, et al.. Establishment of actinorhizal symbiosis in response to ethylene, salicylic acid, and jasmonate. Champion, Antony (ed.); Laplaze, Laurent (ed.). Jasmonate in plant biology : methods and protocols, Humana Press, pp.117-130, 2020, Methods in Molecular Biology, 978-1-0716-0141-9. ⟨10.1007/978-1-0716-0142-6_9⟩. ⟨hal-03283087⟩