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Protein-fragment complementation assays for large-scale analysis of protein-protein interactions

Abstract : Protein-protein interactions (PPIs) orchestrate nearly all biological processes. They are also considered attractive drug targets for treating many human diseases, including cancers and neurodegenerative disorders. Protein-fragment complementation assays (PCAs) provide a direct and straightforward way to study PPIs in living cells or multicellular organisms. Importantly, PCAs can be used to detect the interaction of proteins expressed at endogenous levels in their native cellular environment. In this review, we present the principle of PCAs and discuss some of their advantages and limitations. We describe their application in large-scale experiments to investigate PPI networks and to screen or profile PPI targeting compounds.
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Contributor : Laurent Jonchère Connect in order to contact the contributor
Submitted on : Friday, July 9, 2021 - 9:44:46 AM
Last modification on : Sunday, November 14, 2021 - 6:20:11 PM


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Ewa Blaszczak, Natalia Lazarewicz, Aswani Sudevan, Robert Wysocki, Gwenaël Rabut. Protein-fragment complementation assays for large-scale analysis of protein-protein interactions. Biochemical Society Transactions, Portland Press, 2021, 49 (3), pp.1337-1348. ⟨10.1042/BST20201058⟩. ⟨hal-03282309⟩



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