A microfluidic device to statistically determine the distribution of sickle red cell subpopulations using bioimpedance

Tieying Xu (1) , Maria A Lizarralde-Iragorri (2) , Jean Roman (1) , Emile Martincic (3) , Valentine Brousse (2) , Olivier Français (4) , Wassim El Nemer (2) , Bruno Le Pioufle (5, 6, 7, 1)

1 DALEMBERT - Institut Jean Le Rond d'Alembert
2 BIGR (UMR_S_1134 / U1134) - Biologie Intégrée du Globule Rouge
3 C2N - Centre de Nanosciences et de Nanotechnologies
4 ESYCOM - Electronique, Systèmes de communication et Microsystèmes
5 Université Paris-Saclay
6 LuMIn - Laboratoire Lumière, Matière et Interfaces
7 ENS Paris Saclay - Ecole Normale Supérieure Paris-Saclay

Bioimpedance measurements using microfluidic systems play an increasingly important role in cell analysis. In this paper, a microfluidic device mimicking the vasculature, in particular blood microcapillaries, and equipped with electrodes for bioimpedance sensing is described. Red blood cells, from patients suffering from sickle cell disease, were analyzed and discriminated in this device according to the physical parameters. The statistical position of different subpopulations on the Gaussian distribution of the whole sickle cell population was analyzed. A discrimination between sickle cell subpopulations, related to different density states, was achieved.

Mots clés: Sickle cell subpopulations; Bioimpedance; Microfluidic; Statistical single cell analysis

Domaines: Organisation et fonctions cellulaires [q-bio.SC]

Tieying Xu, Maria A Lizarralde-Iragorri, Jean Roman, Emile Martincic, Valentine Brousse, et al.. A microfluidic device to statistically determine the distribution of sickle red cell subpopulations using bioimpedance. Transducers, 2021, on-line, France. ⟨hal-03260796⟩