A Cellular Potts energy-based approach to analyse the influence of the surface topography on single cell motility
Résumé
cellular scale level, the cell behaviour, especially its migration, is affected by the specificities of the surface
of the substrate, such as the stiffness of the surface and its roughness topography. The latter has been
shown to have a great impact on various cell mechanisms, such as the cell adhesion, migration, or proliferation.
In fact, the mere presence of micro roughness leads to an improvement of those mechanisms,
with a better integration of the implants. However, the phenomena behind those improvements are still
not clear.
In this paper, we propose a three-dimensional (3D) model of a single cell migration using a Cellular
Potts (CP) model to study the influence of the surface topography on cell motility. To do so, various configurations
were tested, such as: (i) a substrate with a random roughness, (ii) a substrate with a rectangular
groove pattern (parallel and perpendicular to the direction of motion), (ii) a substrate with a
sinusoidal groove pattern. To evaluate the influence of the surface topography on cell motility, for each
configuration, the cell speed and shape as well as the contact surface between the cell and the substrate
have been quantified.
Our numerical results demonstrate that, in agreement with the experimental observations of the literature,
the substrate topography has an influence on the cell efficiency (i.e. cell speed), orientation and
shape. Besides, we also show that the increase of the contact surface alone in presence of roughness is
not enough to explain the improvement of cell migration on the various rough surfaces. Finally, we highlight
the importance of the roughness dimension on cell motility. This could be a critical aspect to consider
for further analyses and applications, such as surface treatments for medical applications.
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers produits par l'(les) auteur(s)