A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations]

Ronaldo de Carvalho Augusto (1) , Aki Minoda (2) , Christoph Grunau (1)

1 IHPE - Interactions Hôtes-Pathogènes-Environnements
2 RIKEN IMS - Laboratory for Cardiovascular Genomics and Informatics [Yokohama]

We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size, and it requires little molecular biology handling expertise.

Mots clés: epigenetics; epigenomics; ATAC-seq; Daphnia pulex; Schistosoma mansoni Conceptualization; Investigation; Methodology; Writing -Original Draft Preparation; Writing -Review & Editing; Author roles: Augusto RdC : Conceptualization; Conceptualization; Minoda A Grunau C Funding Acquisition; Project Administration; Writing -Review & Editing No competing interests were disclosed; Schistosoma mansoni

Domaines: Evolution [q-bio.PE]

Ronaldo de Carvalho Augusto, Aki Minoda, Christoph Grunau. A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations]. Wellcome Open Research, 2020, 5, pp.121. ⟨10.12688/wellcomeopenres.15552.1⟩. ⟨hal-03145575v1⟩