Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)

Véronique Vacchina (1) , Katarzyna Bierla (2) , Joanna Szpunar (2) , Ryszard Lobinski (2)

1 UT2A - Ultra Traces Analyses Aquitaine
2 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux

Selenium (Se) is an element readily absorbed during the intestinal tract and distributed in the body. In biological fluids, tissues, and animal products, Se is known to be present mainly in the form of a selenoamino-acid (selenomethionine (SeMet) or selenocysteine (SeCys)). Both amino-acids have different biological activities which justifies their discrimination. Here, we describe the method allowing the simultaneous determination of SeMet and SeCys in blood/plasma, animal tissues, milk, and eggs by twodimensional Liquid Chromatography coupled to Inductively Coupled Plasma Mass Spectrometry (2D HPLC-ICP MS).

Mots clés: Biological fluids and tissues; Selenomethionine; Selenocysteine; HPLC-ICP MS

Domaines: Biochimie [q-bio.BM]

Véronique Vacchina, Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski. Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS). Methods in Molecular Biology, 2017, Selenoproteins. Methods and Protocols, 1661, pp.153 - 162. ⟨10.1007/978-1-4939-7258-6_11⟩. ⟨hal-03133647⟩