Nanoclays for lipase immobilization

H Öztürk , Eric Pollet (1) , V Phalip , Y Güvenilir , Luc Avérous (1)

1 ICPEES - Institut de chimie et procédés pour l'énergie, l'environnement et la santé

© 2016 by the authors. The immobilization of Candida antarctica lipase B (CALB) was performed by physical adsorption on both neat and organo-modified forms of sepiolite and montmorillonite. The influence of different parameters, e.g., solvent, enzyme loading, cross-linking, and type of clay support, on immobilization efficiency and catalyst hydrolytic activity has been investigated. The highest hydrolytic activities were obtained for CALB immobilized on organo-modified clay minerals, highlighting the beneficial effect of organo-modification. The esterification activity of these CALB/organoclay catalysts was also tested in the ring-opening polymerization of "-caprolactone. The polymerization kinetics observed for clay-immobilized catalysts confirmed that CALB adsorbed on organo-modified montmorillonite (CALB/MMTMOD) was the highest-performing catalytic system.

Domaines: Polymères

H Öztürk, Eric Pollet, V Phalip, Y Güvenilir, Luc Avérous. Nanoclays for lipase immobilization. Polymers, 2016, 8 (12), ⟨10.3390/polym8120416⟩. ⟨hal-03115923⟩